Application
Western blot analysis of ASGPR2 expression in A549 whole cell lysate.Application
Western blot analysis of ASGPR2 expression in human fetal lung tissue extract.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human intestine tissue showing cytoplasmic localization.| Product Name | ASGPR2 Antibody (K-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of ASGPR2 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | The Asialoglycoprotein Receptor (ASGPR, also designated hepatic lectin) is a type II integral membrane protein and is expressed in hepatic cells. ASGPR is composed of two homologous subunits, ASGPR1 and ASGPR2, that form multimeric complexes. Both ASGPR1 and ASGPR2 contain four functional domains, which include a cytosolic domain, a transmembrane domain, a stalk domain and a carbohydrate recognition domain (CRD). The CRD allows ASGPR to bind glycoproteins with terminal galactose and N-acetylgalactosamine residues while in the presence of calcium. After binding, the ASGPR-glycoprotein complex is then internalized into the cell, where the receptor and ligand are dissociated and ASGPR returns to the cell membrane. ASGPR can also bind hepatitis B virus (HBV) and mediate the HBV-infection of liver cells. The specific interaction with HBV makes ASGPR a potential target for therapeutic purposes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of ASGPR2 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of ASGPR2 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 433 |
|---|
Application
Western blot analysis of ASGPR2 expression in A549 whole cell lysate.
Application
Western blot analysis of ASGPR2 expression in human fetal lung tissue extract.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human intestine tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
