Application
Western blot analysis of 3β-HSD expression in non-transfected: (A) and human 3β-HSD transfected: (B) 293T whole cell lysates.Application
Western blot analysis of 3β-HSD expression in CCD-1064Sk (A) and MES-SA/Dx5 (B) whole cell lysates.| Product Name | 3β-HSD Antibody (H-143) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 231-373 mapping at the C-terminus of 3β-HSD type I of human origin |
| Modification Notes | 3 beta-hydroxysteroid dehydrogenase (3∫-HSD), also known as HSD3B1 or HSDB3, is a bifunctional enzyme that plays a crucial role in the synthesis of all classes of hormonal steroids. Two human 3∫-HSD proteins, designated type I (3∫-HSD) and type II (3∫-HSD2), are expressed by different genes and function in different areas of the body. Localized to the membrane of the endoplasmic reticulum (ER) and expressed in skin and placenta, 3∫-HSD is the type I protein that catalyzes the oxidative conversion of ∂5-ene-3-beta-hydroxy steroid, as well as the conversion of various ketosteroids. Defects in the gene encoding 3∫-HSD are associated with classic salt wasting, genital ambiguity, hypogonadism, insulin-resistant polycystic ovary syndrome (PCOS) and an increased susceptibility to prostate cancer. Additionally, congenital deficiency of 3∫-HSD activity results in a severe depletion of steroid formation which can be lethal in young children. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of 3β-HSD type I and II of human origin, type I-VI of mouse origin, and type I-IV of rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 231-373 mapping at the C-terminus of 3&beta -HSD type I of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 231-373 mapping at the C-terminus of 3β-HSD type I of human origin |
Application
Western blot analysis of 3β-HSD expression in non-transfected: (A) and human 3β-HSD transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of 3β-HSD expression in CCD-1064Sk (A) and MES-SA/Dx5 (B) whole cell lysates.| Application Notes | recommended for detection of 3β-HSD type I and II of human origin, type I-VI of mouse origin, and type I-IV of rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
