Application
Immunofluorescence staining of methanol-fixed HeLa cells showing mitochondrial localization.Application
Western blot analysis of Blk expression in NAMALWA (A) and Ramos (B) whole cell lysates.| Product Name | Blk Antibody (K-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of Blk of mouse origin |
| Modification Notes | Src is the human homolog of the v-Src gene of the Rous sarcoma virus, also known as avian sarcoma virus or ASV. Src was the first proto-oncogenic non-receptor tyrosine kinase characterized in human. Based on common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Src family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility and adhesion. Src-family kinases contain an amino terminal cell membrane anchor followed by an SH3 domain and an SH2 domain involved in modular association and activation, respectively. Src family kinases are normally maintained in an inactive state and can be activated transiently during cellular events such as mitosis. Different subcellular localizations of Src-family kinases may be important for the regulation of specific cellular processes such as mitogenesis, cyto-skeletal organization and membrane trafficking. The human B lymphocyte kinase gene maps to chromosome 8p23.1 and encodes a 505 amino acid protein, known as Blk. Blk is expressed exclusively by B lymphocytes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Blk of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of Blk of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of Blk of mouse origin |
| Entrez Gene | 640 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing mitochondrial localization.
Application
Western blot analysis of Blk expression in NAMALWA (A) and Ramos (B) whole cell lysates.| Application Notes | recommended for detection of Blk of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
