Application
Western blot analysis of PLEKHG5 expression in Jurkat (A), MCF7 (B) and NTERA-2 cl.D1 (C) whole cell lysates.| Product Name | PLEKHG5 Antibody (N-15) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of PLEKHG5 of human origin by WB, IP, IF and IHC(P) |
| Modification Notes | PLEKHG5 (Pleckstrin homology domain-containing family G member 5), also known as GEF720 (guanine nucleotide exchange factor 720) or DSMA4, is predominantly expressed in the peripheral nervous system and brain. PLEKHG5 localizes to the cytoplasm, however when cells are stimulated, PLEKHG5 is found near the perinuclear regions. PLEKHG5 contains the highly-conserved DH-PH module which is considered to be the signature motif of the Dbl family of guanine nucleotide exchange factors (GEFs). PLEKHG5 activates the NF˚B signaling pathway and may be involved in the control of neuronal cell differentiation. It has been suggested that mutations in the PLEKHG5 gene may lead to autosomal recessive distal spinal muscular atrophy (DSMA). Four isoforms of PLEKHG5 exists due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of PLEKHG5 of human origin by WB IP IF and IHC(P) |
|---|---|
| Tissue Specificity | recommended for detection of PLEKHG5 of human origin by WB, IP, IF and IHC(P) |
| Entrez Gene | 57449 |
|---|
Application
Western blot analysis of PLEKHG5 expression in Jurkat (A), MCF7 (B) and NTERA-2 cl.D1 (C) whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
