Application
Western blot analysis of human recombinant IL-8.| Product Name | IL-8 Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 40-99 mapping at the C-terminus of IL-8 of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | Interleukin-8, or IL-8, the prototypic member of the C-X-C, or a, family of chemokines, is a chemoattractant cytokine involved in the chemotaxis and activation of neutrophils. IL-8 expression has been correlated to a large number of chronic inflammatory diseases, including inflammatory bowel disease (IBD) and atherosclerosis. IL-8 is cleaved from a 99 amino acid precursor to a 72 amino acid, nonglycosylated, biologically active protein. IL-8 monomers and dimers exhibit a dynamic equilibrium both free in solution and in cell surface-bound forms and thus regulate chemotaxis and receptor signaling. Research has shown that IL-8 dimerization functions as a negative regulator for IL-8 receptor function. Two IL-8 receptors, designated IL-8RA and IL-8RB, have been described and share 77% sequence identity. Both are seven-transmembrane domain proteins (7TMD), similar to the G protein-coupled receptors and, in addition to IL-8, serve as receptors for other members of the a and b chemokine families. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIPWB |
| recommended for detection of IL-8 of human origin by WB, IP, IF and ELISA: WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 40-99 mapping at the C-terminus of IL-8 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 40-99 mapping at the C-terminus of IL-8 of human origin |
| Entrez Gene | 3576 |
|---|
Application
Western blot analysis of human recombinant IL-8.| Application Notes | recommended for detection of IL-8 of human origin by WB, IP, IF and ELISA: WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
