Application
Western blot analysis of GTPBP9 expression in non-transfected 293T: (A), mouse GTPBP9 transfected 293T: (B) and Hep G2 (C) whole cell lysates.Application
Western blot analysis of GTPBP9 expression in Hep G2 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human muscle tissue showing cytoplasmic staining.Application
Western blot analysis of GTPBP9 expression in non-transfected 293: (A), human GTPBP9 transfected 293: (B) and Hep G2 (C) whole cell lysates.| Product Name | GTPBP9 Antibody (T-17) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against GTPBP9 of human origin |
| Modification Notes | GTP-binding protein 9 (GTPBP9), also known as Obg-like ATPase 1 (OLA1), is a 396 amino acid protein that belongs to the Obg-related GTPase family under the translation factors (TRAFAC) class. Originally thought to only have GTPase activity, Obg-related GTPase family members have been shown to also have ATPase activity. In Homo sapians, GTPBP9 exhibits a preference for binding ATP over GTP, with GTP binding occuring only at high nucleotide concentration. One cause for ATP affinity and GTP discrimination is thought to be a substitution of glutamine for a hydrophobic amino acid in Obg-related family members; this is the same substitution that inactivates Ras-like GTPases. GTPBP9 contains a C-terminal TGS domain that binds to ligands and an N-terminal G domain which binds nucleotides. GTPBP9 is expressed as three isoforms produced by alternative splicing. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of GTPBP9 of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against GTPBP9 of human origin |
|---|---|
| Tissue Specificity | raised against GTPBP9 of human origin |
| Entrez Gene | 29789 |
|---|
Application
Western blot analysis of GTPBP9 expression in non-transfected 293T: (A), mouse GTPBP9 transfected 293T: (B) and Hep G2 (C) whole cell lysates.
Application
Western blot analysis of GTPBP9 expression in Hep G2 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human muscle tissue showing cytoplasmic staining.
Application
Western blot analysis of GTPBP9 expression in non-transfected 293: (A), human GTPBP9 transfected 293: (B) and Hep G2 (C) whole cell lysates.| Application Notes | recommended for detection of GTPBP9 of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
