Application
Antibodies tested include p-Cortactin (Tyr 466): (A, B, C) and Cortactin (H-191): (D, E, F).Application
Western blot analysis of Cortactin phosphorylation in non-transfected: (A) and human Cortactin transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Cortactin phosphorylation in non-transfected 293T: (A), mouse Cortactin transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of phosphorylated Cortactin expression in untreated (A) and UV-treated (B) HeLa whole cell lysates.| Product Name | p-Cortactin Antibody (Tyr 466) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Tyr 466 of Cortactin of human origin |
| Modification | Phosphoralated |
| Modification Notes | Cortactin (also designated Ems-1) is a filamentous actin (F-actin) binding protein. It has also been shown to be a substrate for Src p60. Cortactin contains tandem 37 amino acid repeats at the amino terminus and an SH3 domain at the carboxy terminus. The tandem repeats appear to be necessary for F-actin binding. Tyrosine phosphorylation of cortactin by Src p60 results in diminished F-actin binding to cortactin and reduced F-actin cross-linking activity. Cortactin has also been shown to be phosphorylated in response to FGF-1. Cortactin exhibits abundant expression in megakaryocytes and platelets, and it may play a role in the maturation of megakaryocytes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| recommended for detection of Tyr 466 phosphorylated Cortactin of mouse origin and correpondingly phosphorylated Tyr 470 of human origin by WB and IP: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Tyr 466 of Cortactin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Tyr 466 of Cortactin of human origin |
| Entrez Gene | 2017 |
|---|
Application
Antibodies tested include p-Cortactin (Tyr 466): (A, B, C) and Cortactin (H-191): (D, E, F).
Application
Western blot analysis of Cortactin phosphorylation in non-transfected: (A) and human Cortactin transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Cortactin phosphorylation in non-transfected 293T: (A), mouse Cortactin transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of phosphorylated Cortactin expression in untreated (A) and UV-treated (B) HeLa whole cell lysates.| Application Notes | recommended for detection of Tyr 466 phosphorylated Cortactin of mouse origin and correpondingly phosphorylated Tyr 470 of human origin by WB and IP: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
