Application
Western blot analysis of APC10 expression in non-transfected 293T: (A), human APC10 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of APC10 expression in non-transfected 293T: (A), mouse APC10 transfected 293T: (B) and HeLa (C) whole cell lysates.| Product Name | APC10 Antibody (FL-185) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-185 representing full length APC10 of human origin |
| Modification Notes | Composed of more than ten subunits, the anaphase-promoting complex (APC) acts in a cell-cycle dependent manner to promote the separation of sister chromatids during the transition between metaphase and anaphase in mitosis. APC, or cyclosome, accomplishes this progression through the ubiquitination of mitotic cyclins and other regulatory proteins that are targeted for destruction during cell division. APC is phosphorylated, and thus activated, by protein kinases Cdk1/cyclin B and polo-like kinase (Plk). APC is under tight control by a number of regulatory factors, including CDC20, CDH1 and MAD2. Specifically, CDC20 and CDH1 directly bind to and activate the cyclin-ubiquitination activity of APCs. In contrast, MAD2 inhibits APC by forming a ternary complex with CDC20 and APC, thus preventing APC activation. APC10 contains a Doc1 homology domain, which is a beta-sandwich structure common to many other putative E3 ubiquitin ligases. APC10 binds to core APC subunits throughout the cell cycle. Specifically, APC10 binds to the C-terminus of CDC27/APC3. During mitosis, APC10 is localized in centrosomes and mitotic spindles. APC10 also localizes to kinetochores from prophase to anaphase, and to the midbody in telophase and cytokinesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of APC10 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-185 representing full length APC10 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-185 representing full length APC10 of human origin |
| Entrez Gene | 10393 |
|---|
Application
Western blot analysis of APC10 expression in non-transfected 293T: (A), human APC10 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of APC10 expression in non-transfected 293T: (A), mouse APC10 transfected 293T: (B) and HeLa (C) whole cell lysates.| Application Notes | recommended for detection of APC10 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
