Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urothelial cancer tissue showing nuclear and cytoplasmic staining of tumor cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of APC7 expression in HeLa (A) and MCF7 (B) nuclear extracts and ZR-75-1 whole cell lysate (C).| Product Name | APC7 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 266-565 of APC7 of human origin |
| Modification Notes | The anaphase-promoting complex (APC) is composed of more than ten subunits, including APC1, APC2, APC4, APC5, APC7, APC8, APC10, and APC11. The APC acts in a cell-cycle dependent manner to promote the separation of sister chromatids during the transition between metaphase and anaphase in mitosis. APC, or cyclosome, accomplishes this progression through the ubiquitination of mitotic cyclins and other regulatory proteins that are targeted for destruction during cell division. APC is phosphorylated, and thus activated, by protein kinases Cdk1/cyclin B and polo-like kinase (Plk). APC is under tight control by a number of regulatory factors, including CDC20, CDH1 and MAD2. Specifically, CDC20 and CDH1 directly bind to and activate the cyclin-ubiquitination activity of APCs. In contrast, MAD2 inhibits APC by forming a ternary complex with CDC20 and APC, thus preventing APC activation. APC7, also known as ANAPC7, is a subunit of APC that mediates the interaction of APC with the transcription coactivators CBP and p300. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of APC7 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 266-565 of APC7 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 266-565 of APC7 of human origin |
| Entrez Gene | 51434 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urothelial cancer tissue showing nuclear and cytoplasmic staining of tumor cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of APC7 expression in HeLa (A) and MCF7 (B) nuclear extracts and ZR-75-1 whole cell lysate (C).| Application Notes | recommended for detection of APC7 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
