Application
Western blot analysis of CENP-H expression in non-transfected: (A) and human CENP-H transfected: (B) 293T whole cell lysates and NIH/3T3 nuclear extract (C).Application
Western blot analysis of CENP-H expression in non-transfected 293T: (A), human CENP-H transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Western blot analysis of CENP-H expression in non-transfected 293T: (A), mouse CENP-H transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of CENP-H expression in non-transfected: (A) and human CENP-H transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CENP-H expression in HeLa whole cell lysate.Application
Western blot analysis of CENP-H expression in HeLa (A), NIH/3T3 (B) and Jurkat (C) nuclear extracts, HeLa whole cell lysate (D) and rat testis (E) and mouse embryo (F) tissue extracts.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Product Name | CENP-H Antibody (FL-247) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-247 representing full length CENP-H of human origin |
| Modification Notes | A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. Both centromere proteins CENP-B and CENP-H are contained in the centromeric heterochromatin between kinetochores, and are involved in maintaining sister chromatid cohesion. The highly dispersed CENP-B promotes and maintaines the joining of DNA satellites in the centromere. CENP-B targets centromeric a-DNA and protects it from digestion by nucleases as well as preventing DNAse or restriction enzyme digestion from affecting the morphology of centromeres. CENP-H contains a coiled-coil structure and a nuclear localization signal. CENP-H is specifically and constitutively localized to kinetochores and plays a role in the organization and function of kinetochores throughout the cell cycle. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CENP-H of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-247 representing full length CENP-H of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-247 representing full length CENP-H of human origin |
| Entrez Gene | 64946 |
|---|
Application
Western blot analysis of CENP-H expression in non-transfected: (A) and human CENP-H transfected: (B) 293T whole cell lysates and NIH/3T3 nuclear extract (C).
Application
Western blot analysis of CENP-H expression in non-transfected 293T: (A), human CENP-H transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Western blot analysis of CENP-H expression in non-transfected 293T: (A), mouse CENP-H transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of CENP-H expression in non-transfected: (A) and human CENP-H transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CENP-H expression in HeLa whole cell lysate.
Application
Western blot analysis of CENP-H expression in HeLa (A), NIH/3T3 (B) and Jurkat (C) nuclear extracts, HeLa whole cell lysate (D) and rat testis (E) and mouse embryo (F) tissue extracts.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Application Notes | recommended for detection of CENP-H of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
