Application
Western blot analysis of MPO and MPO heavy chain expression in HL-60 whole cell lysate.| Product Name | MPO heavy chain Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 446-745 mapping at the C-terminus of MPO of human origin |
| Modification Notes | The heme protein myeloperoxidase (MPO) is a major component of azurophilic granules of neutrophils and polymorphonuclear leukocytes. Optimal oxygen-dependent microbiocidal activity depends on MPO as the critical enzyme for the generation of hypochlorous acid and other toxic oxygen products. The MPO precursor is synthesized during the promyelocytic stage of myeloid differentiation and is subsequently processed and transported intracellularly to the lysosomes. The precursor undergoes cotranslational N-linked glycosylation to produce a glycoprotein. Glucosidases in the endoplasmic reticulum (ER) or early cis Golgi convert the pro-MPO to a form which is sorted into a prelysosomal compartment, which undergoes final proteolytic maturation to native MPO, a pair of heavy-light protomers. In normal neutrophils, MPO is expressed as a dimer. Calreticulin, a calcium-binding protein residing in the ER, interacts specifically with fully glycosylated apopro-MPO. iMPO mRNA is abundant in human promyelocytic HL-60 and mouse myeloid leukemia NFS-60 cells. MPO is expressed at high levels in circulating neutrophils and monocytes but is not detectable in microglia, brain-specific macrophages or normal brain tissue. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MPO heavy chain, eosinophil peroxidase, and, to a lesser extent, lactoperoxidase, and thyroid peroxidase of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 446-745 mapping at the C-terminus of MPO of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 446-745 mapping at the C-terminus of MPO of human origin |
| Entrez Gene | 4353 |
|---|
Application
Western blot analysis of MPO and MPO heavy chain expression in HL-60 whole cell lysate.| Application Notes | recommended for detection of MPO heavy chain, eosinophil peroxidase, and, to a lesser extent, lactoperoxidase, and thyroid peroxidase of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
