Application
Western blot analysis of FXYD3 expression in non-transfected: (A) and mouse FXYD3 transfected: (B) 293T whole cell lysates.| Product Name | FXYD3 Antibody (M-88) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-88 representing full length FXYD3 of mouse origin |
| Modification Notes | The mammalian FXYD family maintains Na+ and K+ gradients between the intracellular and extracellular milieus of cells in processes such as renal Na+-reabsorption, muscle contraction and neuronal excitability. FXYDs are single-span membrane proteins that share a 35 amino acid signature domain, beginning with the sequence PFXYD and containing seven invariant and six conserved amino acids. Members of the FXYD family include FXYD1 (PLM, phospholemman), FXYD2 (the © subunit of the Na/K-ATPase), FXYD3 (Mat8, mammary tumor protein), FXYD4 (CHIF) and FXYD5 (RIC). FXYD3, a 67 amino acid protein, may act as a chloride channel or as a chloride channel regulator. It is expressed in a subset of human breast tumors and shows partial homology to FXYD1. FXYD3 has a putative 20 amino acid leader sequence and a putative transmembrane domain (with two cysteine residues). It contains no consensus phosphorylation sites in the cytoplasmic domain. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of FXYD3 and lesser reactivity with FXYD4 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-88 representing full length FXYD3 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-88 representing full length FXYD3 of mouse origin |
| Entrez Gene | 5349 |
|---|
Application
Western blot analysis of FXYD3 expression in non-transfected: (A) and mouse FXYD3 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of FXYD3 and lesser reactivity with FXYD4 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
