Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of cyclin D3 expression in Jurkat whole cell lysate.Application
Western blot analysis of cyclin D3 expression in non-transfected: (A) and mouse cyclin D3 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of cyclin D3 expression in HeLa (A), K-562 (B) and Jurkat (C) phorbol ester-induced nuclear extracts.| Product Name | cyclin D3 Antibody (C-16) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of cyclin D3 of human origin |
| Modification Notes | The proliferation of eukaryotic cells is controlled at specific points in the cell cycle, particularly at the G1 to S and the G2 to M transitions. It is well established that the Cdc2 p34-cyclin B protein kinase plays a critical role in the G2 to M transition while cyclin A associates with Cdk2 p33 and functions in S phase. Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl-1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of cyclin D3 p34 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA non cross-reactive with cyclin D1 or cyclin D2 also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of cyclin D3 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of cyclin D3 of human origin |
| Entrez Gene | 896 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of cyclin D3 expression in Jurkat whole cell lysate.
Application
Western blot analysis of cyclin D3 expression in non-transfected: (A) and mouse cyclin D3 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of cyclin D3 expression in HeLa (A), K-562 (B) and Jurkat (C) phorbol ester-induced nuclear extracts.| Application Notes | recommended for detection of cyclin D3 p34 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA non cross-reactive with cyclin D1 or cyclin D2 also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
