Application
Western blot analysis of AICAR expression in non-transfected 293T: (A), human AICAR transfected 293T: (B) and CCRF-CEM (C) whole cell lysates.Application
Western blot analysis of AICAR transformylase expression in CCRF-CEM whole cell lysate (A) and human recombinant AICAR transformylase fusion protein (B).Application
Western blot analysis of AICAR transformylase expression in non-transfected 293T: (A), mouse AICAR transformylase transfected 293T: (B) and COLO 320DM (C) whole cell lysates.| Product Name | AICAR transformylase Antibody (H-270) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-270 mapping at the N-terminus of AICAR transformylaseof human origin |
| Modification Notes | The bifunctional purine biosynthesis protein ATIC (also designated PURH) contains AICAR transformylase and IMP cyclohydrolase activities. AICAR (5-aminoimidazole-4-carboxamide ribonucleotide) transformylase catalyzes the second to last step in purine biosynthesis, playing an important role in the production of nucleotides and IMP. Defects in the ATIC transformylase gene can cause AICA-rebsuria, also designated AICA-ribosiduria, an inborn error in purine biosynthesis that is neurologically cataclysmic. Individuals with AICA-rebosuria accumulate AICA-riboside, also designated ZMP, and its derivatives in erythrocytes and fibroblasts. Patients also excrete very large amounts of AICA-riboside in the urine. Mental retardation, epilepsy, dysmorphic features and congenital blindness are all symptoms of this disease. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of AICAR transformylase of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-270 mapping at the N-terminus of AICAR transformylaseof human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-270 mapping at the N-terminus of AICAR transformylaseof human origin |
| Entrez Gene | 471 |
|---|
Application
Western blot analysis of AICAR expression in non-transfected 293T: (A), human AICAR transfected 293T: (B) and CCRF-CEM (C) whole cell lysates.
Application
Western blot analysis of AICAR transformylase expression in CCRF-CEM whole cell lysate (A) and human recombinant AICAR transformylase fusion protein (B).
Application
Western blot analysis of AICAR transformylase expression in non-transfected 293T: (A), mouse AICAR transformylase transfected 293T: (B) and COLO 320DM (C) whole cell lysates.| Application Notes | recommended for detection of AICAR transformylase of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
