Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomeruli and tubules at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of CHMP5 expression in HEL 92.1.7 (A), SK-N-SH (B) and K-562 (C) whole cell lysates.| Product Name | CHMP5 Antibody (H-90) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against amino acids 130-219 mapping at the C-terminus of CHMP5 of human origin |
| Modification Notes | The charged multivesicular body proteins, commonly designated CHMPs, belong to the vacuolar sorting protein family and function as chromatin-modifying proteins. CHMP1-6 are all components of ESCRT (endosomal sorting complex required for transport) I, II or III complexes. These complexes are crucial for sorting endosomal articles into multivesicular bodies (MVBs) and are required for the formation of these bodies. During HIV-1 infection, the virus uses the ESCRT-III complex to mediate budding and exocytosis of viral proteins. CHMP5 interacts directly with LIP5, a protein re-quired for HIV release. Depletion of LIP5 will reduce HIV-1 budding, where-as a depletion of CHMP5 will increase HIV-1 release. Subsequently, over-expression of CHMP5 will reduce HIV-1 budding. CHMP5 also regulates late endosomal development downstream of MVB formation and a loss of CHMP5 will result in increased signal transduction due to a decrease in lysosomal degradation function. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CHMP5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against amino acids 130-219 mapping at the C-terminus of CHMP5 of human origin |
|---|---|
| Tissue Specificity | raised against amino acids 130-219 mapping at the C-terminus of CHMP5 of human origin |
| Entrez Gene | 51510 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomeruli and tubules at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of CHMP5 expression in HEL 92.1.7 (A), SK-N-SH (B) and K-562 (C) whole cell lysates.| Application Notes | recommended for detection of CHMP5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
