Application
Western blot analysis of Histone H2A.Z/H2A.F/Z expression in mouse placenta (A) and rat placenta (B) tissue extracts.Application
Western blot analysis of Histone H2A.Z expression in non-transfected 293T: (A), human Histone H2A.Z transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Histone H2A.F/Z expression in non-transfected: (A) and human Histone H2A.F/Z transfected: (B) 293T whole cell lysates.| Product Name | Histone H2A.Z/H2A.F/Z Antibody (FL-128) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-128 representing full length histone H2A.Z of human origin |
| Modification Notes | Histone H2A.Z/H2A.F/Z (H2A/z) is a 128 amino acid protein encoded by the human gene H2AFZ. Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA sequentially in a left-handed super-helical turn to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3 and H4) form the octamer, which is comprised of two H2A-H2B dimers and two H3-H4 dimers, creating two nearly symmetrical halves by tertiary structure. H2A.Z/H2A.F/Z is a variant Histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in tran-scription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of posttranslational modifications of histones, also called histone code, and nucleosome remodeling. H2A.Z/H2A.F/Z may be involved in the formation of constitutive heterochromatin and may be required for chromosome segregation during cell division. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of histone H2A.Z and histone H2A.F/Z of mouse, rat and human origin by WB, IP, IF and ELISA may cross-react with other histone H2A family members also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-128 representing full length histone H2A.Z of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-128 representing full length histone H2A.Z of human origin |
| Entrez Gene | 3015 |
|---|
Application
Western blot analysis of Histone H2A.Z/H2A.F/Z expression in mouse placenta (A) and rat placenta (B) tissue extracts.
Application
Western blot analysis of Histone H2A.Z expression in non-transfected 293T: (A), human Histone H2A.Z transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Histone H2A.F/Z expression in non-transfected: (A) and human Histone H2A.F/Z transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of histone H2A.Z and histone H2A.F/Z of mouse, rat and human origin by WB, IP, IF and ELISA may cross-react with other histone H2A family members also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
