Application
Western blot analysis of JMY expression in non-transfected 293T: (A), mouse JMY transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing perinuclear staining.| Product Name | JMY Antibody (M-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of JMY of mouse origin |
| Modification Notes | p300 and CBP (CREB-binding proteins) function as coactivators for various transcription factors, including p53. As cofactors, p300 and CBP possesses intrinsic acetyltransferase activity which may allow p300/CBP proteins to regulate transcription through direct acetylation and thereafter, enhance DNA binding activity. JMY is a nuclear cofactor for p300 that cooperatively enhances p53 activation in response to cellular stress. The p53 protein requires p300/CBP coactivator proteins in order to transcriptionally activate target genes. When p53 is activated, p300 component of the coactivator protein complexes associate with JMY and potentiate p53-dependent transcription and apoptosis. p53 acts as a sequence-specific transcription factor and upon stimulation, induces transcription of genes involved in growth arrest, including the waf1/cip1, bax, mdm2, and gadd45 genes. Disruption of p300 and JMY complexes inhibits p53-induced transcription of bax and blocks apoptosis. Due to alternative splicing, several isoforms of JMY are produced, and these various isoforms have different influencing affects on p53 activation, with some isoforms markedly enhancing p53 responses compared to the other splicing variants. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of JMY of mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of JMY of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of JMY of mouse origin |
| Entrez Gene | 57748 |
|---|
Application
Western blot analysis of JMY expression in non-transfected 293T: (A), mouse JMY transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing perinuclear staining.| Application Notes | recommended for detection of JMY of mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
