Application
Western blot analysis of IMPDH2 expression in non-transfected: (A) and mouse IMPDH2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of IMPDH2 expression in non-transfected: (A) and mouse IMPDH2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of IMPDH expression in non-transfected: (A) and human IMPDH transfected: (B) 293T whole cell lysates.| Product Name | IMPDH Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 215-514 mapping at the C-terminus of IMPDH1 of human origin |
| Modification Notes | A member of the GMPR family, inosine-5'-monophosphate dehydrogenase 1 (IMPDH1) functions in the regulation of cell growth by catalyzing the rate-limiting step in the de novo synthesis of guanine nucleotides. IMPDH1 is a ubiquitously expressed homotetramer that plays an important role in cyclic nucleoside metabolism within photoreceptors. Expression of IMPDH1 is the main type found in normal leukocytes, while IMPDH2 predominates in tumors. Mutations in IMPDH1 are associated with the autosomal dominant retinitis pigmentosa type 10 (RP10), as well as the development of malignant tumors. Analysis of mutant IMPDH1 suggests that protein misfolding and aggregation leads to the severe phenotype rather than reduced IMPDH1 activity. Therefore, IMPDH1 may be a potential therapeutic target based upon a strategy combining simultaneous suppression of IMPDH1 transcripts with supplementation of GTP within retinal tissues. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of IMPDH1 and IMPDH2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 215-514 mapping at the C-terminus of IMPDH1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 215-514 mapping at the C-terminus of IMPDH1 of human origin |
| Entrez Gene | 3614 |
|---|
Application
Western blot analysis of IMPDH2 expression in non-transfected: (A) and mouse IMPDH2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of IMPDH2 expression in non-transfected: (A) and mouse IMPDH2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of IMPDH expression in non-transfected: (A) and human IMPDH transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of IMPDH1 and IMPDH2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
