Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing nuclear envelope and cytoplasmic staining of myocytes.Application
Western blot analysis of Lamin B1 expression in Jurkat (A) and Y79 (B) whole cell lysates.Application
Immunofluorescence staining of normal mouse lymph node frozen section showing nuclear envelope staining.Application
Immuno?fluorescence staining of methanol-fixed HeLa cells showing nuclear envelope localization.| Product Name | Lamin B1 Antibody (H-90) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 401-490 of Lamin B1 of human origin |
| Modification Notes | A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, termed Ced-3/ICE, function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Nuclear Lamins are critical to maintaining the integrity of the nuclear envelope and cellular morphology as components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. B-type Lamins, such as Lamin B1, undergo a series of modifications, such as farnesylation and phosphorylation. Lamin B1 is a 586 amino acid protein that is encoded by a gene which, when mutated, is involved in the pathogenesis of autosomal dominant adult-onset leukodystrophy (ADLD), a disease characterized by cerebellar dysfunction and symmetric demyelination of the central nervous system. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Lamin B1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 401-490 of Lamin B1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 401-490 of Lamin B1 of human origin |
| Entrez Gene | 4001 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing nuclear envelope and cytoplasmic staining of myocytes.
Application
Western blot analysis of Lamin B1 expression in Jurkat (A) and Y79 (B) whole cell lysates.
Application
Immunofluorescence staining of normal mouse lymph node frozen section showing nuclear envelope staining.
Application
Immuno?fluorescence staining of methanol-fixed HeLa cells showing nuclear envelope localization.| Application Notes | recommended for detection of Lamin B1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
