| Product Name | p-Stat5a Antibody (Ser 127/128) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to dually phosphorylated Ser 127 and 128 of Stat5a of human origin |
| Modification | Phosphoralated |
| Modification Notes | Stat5 (Signal Transducers and Activators of Transcription 5) is important in regulating T cell functions involving the receptors for Interleukin-2 (IL-2) (1). IL-2 stimulates the rapid phosphorylation of both serine and tyrosine residues of Stat5a and Stat5b in human T lymphocytes and in several IL-2-responsive lymphocytic cell lines (2–4). IL-2 differentially induces serine phosphorylation of Stat5a and Stat5b on Ser726 and Ser731, respectively (2,5). Stat5b is preferentially phosphorylated and displays more protracted serine phosphorylation kinetics than Stat5a (2,5). Both the acid-rich region and the COOH terminus of IL-2Rb can independently mediate IL-2-induced Stat 5a/b serine phosphorylation, suggesting that Stat5a/b serine phosphorylation occurs at a postreceptor level (2). Stat5a is phosphorylated on Tyr694 in a prolactin-sensitive manner, whereas serine phosphorylation is constitutive. Activation of Stat5 by IL-2 may help govern the biological effects of IL-2 during the immune response (3). Ser779 is constitutively phosphorylated in the mammary gland, and Ser725 phosphorylation influences prolactin-stimulated in vitro DNA binding activity. Phosphorylation of Stat5a at Ser-127/Ser-128 is required for the regulation of ErbB4-mediated activation of Stat5a. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIFELISA |
| recommended for detection of Ser 127 and 128 dually phosphorylated Stat5a of mouse, rat and human origin by WB, IF and ELISA may cross-react with correspondingly phosphorylated Stat5b: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to dually phosphorylated Ser 127 and 128 of Stat5a of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to dually phosphorylated Ser 127 and 128 of Stat5a of human origin |
| Entrez Gene | 6776 |
|---|
| Application Notes | recommended for detection of Ser 127 and 128 dually phosphorylated Stat5a of mouse, rat and human origin by WB, IF and ELISA may cross-react with correspondingly phosphorylated Stat5b: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
