Application
Western blot analysis of RNF25 expression in mouse prostate tissue extract.Application
Western blot analysis of RNF25 expression in Hep G2 whole cell lysate.Application
Immuno-peroxidase staining of formalin fixed, paraffin-embedded human skin tissue showing nuclear and cytoplasmic staining.| Product Name | RNF25 Antibody (T-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against RNF25 of human origin |
| Modification Notes | Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). RNF25 (RING finger protein 25), also known as AO7, is a 459 amino acid protein that contains one RWD domain and one RING-type zinc finger. Via its RING-type zinc finger (a common domain that contains a conserved cysteine-rich region and is found in a number of viral and eukaryotic proteins), RNF25 is thought to exhibit E2-dependent E3 ubiquitin-protein ligase activity. Specifically, the RING finger of RNF25 interacts with an E2 ligase and, through this interaction, facilitates the ubiquitination of target proteins. RNF25 may also regulate the transcriptional activity of NF˚B p65, a protein that plays important roles in cell survival, inflammation response, host defense and proliferation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of RNF25 of human and canine origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against RNF25 of human origin |
|---|---|
| Tissue Specificity | raised against RNF25 of human origin |
| Entrez Gene | 64320 |
|---|
Application
Western blot analysis of RNF25 expression in mouse prostate tissue extract.
Application
Western blot analysis of RNF25 expression in Hep G2 whole cell lysate.
Application
Immuno-peroxidase staining of formalin fixed, paraffin-embedded human skin tissue showing nuclear and cytoplasmic staining.| Application Notes | recommended for detection of RNF25 of human and canine origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
