Application
Western blot analysis of PC2 expression in non-transfected: (A) and human PC2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PC2 expression in SK-N-SH whole cell lysate.| Product Name | PC2 Antibody (H-68) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 571-638 mapping at the C-terminus of PC2 of human origin |
| Modification Notes | The subtilisin-like prohormone convertase (PC) family mediates the cleavage of latent precursor proteins into their biologically active forms. This is a tightly regulated process that leads to the generation of various active peptides and proteins, including neuropeptides, polypeptide hormones, protein tyrosine phosphatases, growth factors and their receptors, and enzymes such as matrix metalloproteases (MMPs). These processing reactions occur at pairs of basic amino acids. The members of the PC family include furin, PC1/3, PC2, PC4, PACE4, PC5/6, and PC7/8 (also designated lymphoma proprotein convertase or LPC), all of which share homology to the bacterial subtilisin and yeast kexin families of endoproteases. PC1/3, also designated neuroendocrine 1 (NEC1), and PC2, also designated neuroendocrine 2 (NEC2), are widely expressed in neuroendocrine tissues, and are principally involved in the processing of hormonal and neural peptides. The human PC2 gene maps to chromosome 20p11, and is expressed in pancreatic islets, pituitary, and brain as a precursor protein and a mature form. Cleavage of proPC2 is dependent upon its interaction with 7B2, a cofactor that acts as both an activator and inhibitor of PC2 function. Proteins processed by PC2 include proglucagon, prosomatostatin, proinsulin, and pro-islet amyloid polypeptide. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PC2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 571-638 mapping at the C-terminus of PC2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 571-638 mapping at the C-terminus of PC2 of human origin |
| Entrez Gene | 5126 |
|---|
Application
Western blot analysis of PC2 expression in non-transfected: (A) and human PC2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PC2 expression in SK-N-SH whole cell lysate.| Application Notes | recommended for detection of PC2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
