Application
Western blot analysis of Daxx expression in Jurkat whole cell lysate.Application
Western blot analysis of Daxx expression in PC-12 (A) and 3611-RF (B) whole cell lysates.Application
Western blot analysis of Daxx expression in HeLa (A), BJAB (B), Ramos (C), K-562 (D), RAW 264.7 (E) and WEHI-231 (F) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed RAW 264.7 cells showing nuclear localization.| Product Name | Daxx Antibody (M-112) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 627-739 mapping at the C-terminus of Daxx of mouse origin |
| Modification Notes | Activation of the cell surface receptor FAS by FAS ligand leads to the initiation of apoptosis, a process necessary for the regulation of the immune system and tissue homeostasis. FAS-mediated apoptosis appears to involve a number of divergent and overlapping pathways. Daxx appears to be a central component of a FAS-mediated apoptotic pathway involving the activation of Jun N-terminal kinase (JNK). Although Daxx itself does not contain a death domain, it specifically binds to the death domain of FAS. Overexpression of Daxx activates the JNK pathway and enhances FAS-mediated apoptosis. The Daxx apoptotic pathway acts cooperatively with but is distinct from the FAS-mediated pathway that involves interactions between the death domain-containing protein FADD and the cysteine protease FLICE. Unlike the FAS-FADD-FLICE pathway, the Daxx pathway is sensitive to the apoptotic inhibitor protein Bcl-2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Daxx of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 627-739 mapping at the C-terminus of Daxx of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 627-739 mapping at the C-terminus of Daxx of mouse origin |
| Entrez Gene | 1616 |
|---|
Application
Western blot analysis of Daxx expression in Jurkat whole cell lysate.
Application
Western blot analysis of Daxx expression in PC-12 (A) and 3611-RF (B) whole cell lysates.
Application
Western blot analysis of Daxx expression in HeLa (A), BJAB (B), Ramos (C), K-562 (D), RAW 264.7 (E) and WEHI-231 (F) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed RAW 264.7 cells showing nuclear localization.| Application Notes | recommended for detection of Daxx of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
