Application
Western blot analysis of NCoA-3 expression in Jurkat (A) and K-562 (B) whole cell lysates.Application
Western blot analysis of NCoA-3 expression in HeLa whole cell lysate.Application
Immunofluorescence staining of normal mouse liver frozen section showing nuclear staining.Application
Control Input (A). Antibodies used included SRC-1 (M-341): , SRC-1 (C-20): and SRC-1 (M-20): (B), Grip-1 (M-434): (C), NCoA-3 (F-2): , NCoA-3 (M-397): , NCoA-3 (N-17): and NCoA-3 (C-20): (D). Data kindly provided by M.G. Rosenfeld and reproduced wtih permission from Gold et al., Neuron 2003, 40: 1119-1131.Application
Antibodies tested include NCoA-3 (F-2): , NCoA-3 (M-397): , NCoA-3 (N-17): , NCoA-3 (C-20): , SRC-1 (M-341): , SRC-1 (C-20): , SRC-1 (M-20): , GRIP1 (M-343): , PCAF (C-16): and PCAF (H-369): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.Application
Antibodies tested include p300 (C-20): 585, p300 (N-15): 584, p300 (H-272): 8981, NCoA-3 (F-2): 5305, NCoA-3 (M-397): 9119, NCoA-3 (N-17): 7217, NCoA-3 (C-20): 7216, SRC-1 (M-341): 8995, SRC-1 (C-20): 6096, SRC-1 (M-20): 6098 and GRIP1 (M-343): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.Application
Antibodies tested include ERα (MC-20): , ERα (H-184): , CBP (A-22): , CBP (C-1): , p/CIP (F2): , p/CIP (M-397): , RIP140 (H-300): and HDAC3 (N-19): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Perissi et al., Cell 2004, 116: 511-526.Application
Western blot analysis of NCoA-3 expression in K-562 whole cell lysate.Application
Western blot analysis of His-tagged mouse recombinant NCoA-3.| Product Name | NCoA-3 Antibody (M-397) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 455-851 mapping within an internal region of NCoA-3 of mouse origin |
| Modification Notes | Nuclear receptors for steroids, thyroid hormones and retinoic acids are ligand-dependent transcription factors that activate transcription through specific DNA binding sites in their target genes. Several related transcriptional coactivators and corepressors have been described that work in concert with the steroid receptor family to either induce or repress transcription from hormone-responsive elements. This family includes GRIP1 (for GR interacting protein 1), also designated NCoA-2 or TIF-2; SRC-1 (for steroid receptor coactivator-1), also designated NCoA-1; and NCoA-3, also designated RAC3, ACTR, AIB-1 (for amplified in breast cancer) and p/CIP (for p300/CBP/co-integrator protein), which displays elevated expression in estrogen receptor positive ovarian and breast cancers and is required for the transcriptional activation of p300/CBP-dependent transcription factors. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAChIP |
| recommended for detection of NCoA-3 and, to a lesser extent, GRIP-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 455-851 mapping within an internal region of NCoA-3 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 455-851 mapping within an internal region of NCoA-3 of mouse origin |
| Entrez Gene | 8202 |
|---|
Application
Western blot analysis of NCoA-3 expression in Jurkat (A) and K-562 (B) whole cell lysates.
Application
Western blot analysis of NCoA-3 expression in HeLa whole cell lysate.
Application
Immunofluorescence staining of normal mouse liver frozen section showing nuclear staining.
Application
Control Input (A). Antibodies used included SRC-1 (M-341): , SRC-1 (C-20): and SRC-1 (M-20): (B), Grip-1 (M-434): (C), NCoA-3 (F-2): , NCoA-3 (M-397): , NCoA-3 (N-17): and NCoA-3 (C-20): (D). Data kindly provided by M.G. Rosenfeld and reproduced wtih permission from Gold et al., Neuron 2003, 40: 1119-1131.
Application
Antibodies tested include NCoA-3 (F-2): , NCoA-3 (M-397): , NCoA-3 (N-17): , NCoA-3 (C-20): , SRC-1 (M-341): , SRC-1 (C-20): , SRC-1 (M-20): , GRIP1 (M-343): , PCAF (C-16): and PCAF (H-369): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.
Application
Antibodies tested include p300 (C-20): 585, p300 (N-15): 584, p300 (H-272): 8981, NCoA-3 (F-2): 5305, NCoA-3 (M-397): 9119, NCoA-3 (N-17): 7217, NCoA-3 (C-20): 7216, SRC-1 (M-341): 8995, SRC-1 (C-20): 6096, SRC-1 (M-20): 6098 and GRIP1 (M-343): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.
Application
Antibodies tested include ERα (MC-20): , ERα (H-184): , CBP (A-22): , CBP (C-1): , p/CIP (F2): , p/CIP (M-397): , RIP140 (H-300): and HDAC3 (N-19): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Perissi et al., Cell 2004, 116: 511-526.
Application
Western blot analysis of NCoA-3 expression in K-562 whole cell lysate.
Application
Western blot analysis of His-tagged mouse recombinant NCoA-3.| Application Notes | recommended for detection of NCoA-3 and, to a lesser extent, GRIP-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
