Application
Western blot analysis of TRα/β expression in C32 nuclear extract (A) and C32 whole cell lysate (B).| Product Name | TRα/β Antibody (FL-408) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-408 representing the full length TRα1 of avian origin |
| Modification Notes | Thyroid hormone nuclear receptors (TRs) are ligand- dependent transcription factors which regulate growth, differentiation and development and represent members of the steroid/retinoic acid superfamily. The two genes encoding TRs identified to date, TRå and TR∫, have been mapped to human chromosomes 17 and 3, respectively. TRs bind to thyroid hormone response elements (TREs) with half site binding motifs in the orientation of palindromes, direct repeats or inverted palindromes. The affinities of binding are both variable and influenced differentially by 3,5,3’-triiodo-L-thyronine (T3). Transcriptional regulation by TRs is also modulated by heterodimerization with TR nuclear accessory proteins, the most extensively characterized of which are the retinoid X receptors (RXRå, RXR∫ and RXR©). To a certain extent, this activity is regulated by differential phosphorylation of TRs. Thus, not only are the biological activities of TRs regulated by heterodimerization with RXRs, but in addition, the gene regulatory activities of TRs are linked to other hormonal pathways. TRå1 can display both a nuclear and undefined cytoplasmic location, and is the only TR that is imported into the mitochondrial matrix. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of TRα1, TRα2, TRα3, TRβ1 and TRβ2 of mouse, rat, human and avian origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-408 representing the full length TR&alpha 1 of avian origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-408 representing the full length TRα1 of avian origin |
| Entrez Gene | 7067 |
|---|
Application
Western blot analysis of TRα/β expression in C32 nuclear extract (A) and C32 whole cell lysate (B).| Application Notes | recommended for detection of TRα1, TRα2, TRα3, TRβ1 and TRβ2 of mouse, rat, human and avian origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
