Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of CRMP-4 expression in non-transfected: (A) and human CRMP-4 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CRMP-1 expression in non-transfected: (A) and human CRMP-1 transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic and membrane staining of decidual and trophoblastic cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of CRMP-2 expression in non-transfected: (A) and human CRMP-2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CRMP-4 expression in non-transfected 293T: (A), mouse CRMP-4 transfected 293T: (B) and SK-N-MC (C) whole cell lysates.Application
Western blot analysis of CRMP expression in IMR-32 whole cell lysate.Application
Western blot analysis of CRMP-2 expression in non-transfected: (A) and human CRMP-2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CRMP-4 expression in non-transfected: (A) and human CRMP-4 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CRMP-4 expression in non-transfected: (A) and human CRMP-4 transfected: (B) 293T whole cell lysates and rat brain tissue extract (C).Application
Western blot analysis of CRMP expression in HUV-EC-C whole cell lysate (A) and mouse lung tissue extract (B).Application
Western blot analysis of CRMP expression in Jurkat whole cell lysate.Application
Western blot analysis of CRMP-4 expression in non-transfected: (A) and human CRMP-4 transfected: (B) 293T whole cell lysates and rat brain tissue extract (C).| Product Name | CRMP Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 273-572 mapping at the C-terminus of CRMP-2 of human origin |
| Modification Notes | Collapsin response mediator proteins (CRMPs), including CRMP-1 (DRP-1), CRMP-2 (DRP-2 or TOAD64), CRMP-3 (DRP-4), CRMP-4 (DRP-3) and CRMP-5 (DRP-5), mediate signal transduction after exposure of neural cells to the axon guidance molecule Semaphorin 3A (SEMA3A)/collapsin. CRMPs are present in the developing cerebral cortex and neocortical neurons and are responsive to SEMA3A. In the adult brain, the expression of CRMPs is dramatically downregulated. However, they remain expressed in structures that retain their capacity for differentiation and plasticity. CRMP-2 is involved in axonal growth and guidance. The human CRMP-2 gene is located in 8p22-p21, a chromosomal region that has been previously shown to have a significant linkage to schizophrenia and to several deficit symptoms of schizophrenia. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CRMP-2, CRMP-1, CRMP-3, CRMP-4 and, to a lesser extent, CRMP-5 and DHP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 273-572 mapping at the C-terminus of CRMP-2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 273-572 mapping at the C-terminus of CRMP-2 of human origin |
| Entrez Gene | 1400 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of CRMP-4 expression in non-transfected: (A) and human CRMP-4 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CRMP-1 expression in non-transfected: (A) and human CRMP-1 transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic and membrane staining of decidual and trophoblastic cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of CRMP-2 expression in non-transfected: (A) and human CRMP-2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CRMP-4 expression in non-transfected 293T: (A), mouse CRMP-4 transfected 293T: (B) and SK-N-MC (C) whole cell lysates.
Application
Western blot analysis of CRMP expression in IMR-32 whole cell lysate.
Application
Western blot analysis of CRMP-2 expression in non-transfected: (A) and human CRMP-2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CRMP-4 expression in non-transfected: (A) and human CRMP-4 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CRMP-4 expression in non-transfected: (A) and human CRMP-4 transfected: (B) 293T whole cell lysates and rat brain tissue extract (C).
Application
Western blot analysis of CRMP expression in HUV-EC-C whole cell lysate (A) and mouse lung tissue extract (B).
Application
Western blot analysis of CRMP expression in Jurkat whole cell lysate.
Application
Western blot analysis of CRMP-4 expression in non-transfected: (A) and human CRMP-4 transfected: (B) 293T whole cell lysates and rat brain tissue extract (C).| Application Notes | recommended for detection of CRMP-2, CRMP-1, CRMP-3, CRMP-4 and, to a lesser extent, CRMP-5 and DHP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
