Application
Western blot analysis of PACSIN1 expression in non-transfected: (A) and mouse PACSIN1 transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic staining of cells in molecular and granular layers and Purkinje cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.| Product Name | PACSIN1 Antibody (M-46) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 301-346 mapping within an internal region of PACSIN1 of mouse origin |
| Modification Notes | PACSINs are members of a family of cytoplasmic adapter proteins, which share a conserved C-terminal protein binding SH3 domain and a CDC15-NT domain (1,2). PACSIN 1-related proteins include syndapin 1 (the rat homolog of PACSIN 1), FAP52, EM13, and PSTPIP, all of which seem to be involved in signaling pathways associated with cytoskeletal organization (3-6). PACSIN 1 expression is restricted to terminally differentiated neural tissue, whereas PACSIN 2 is widely expressed (1). PACSIN 2 shows vesicle-like distribution and may be involved in regulating endocytotic processes (2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PACSIN1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 301-346 mapping within an internal region of PACSIN1 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 301-346 mapping within an internal region of PACSIN1 of mouse origin |
| Entrez Gene | 29993 |
|---|
Application
Western blot analysis of PACSIN1 expression in non-transfected: (A) and mouse PACSIN1 transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic staining of cells in molecular and granular layers and Purkinje cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.| Application Notes | recommended for detection of PACSIN1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
