Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.Application
Western blot analysis of ChREBP expression in HeLa whole cell lysate.| Product Name | ChREBP Antibody (M-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 401-700 mapping within an internal region of ChREBP of mouse origin |
| Modification Notes | ChREBP (for carbohydrate responsive binding protein, also designated Mlx interactor, WBSCR14 and MondoB) is a transcription factor that binds to the carbohydrate-responsive element of the L-type pyruvate kinase gene (L-PK). ChREBP is expressed specifically in liver and is activated by high glucose and inhibited by cAMP or a high fat diet. ChREBP is likely critical for the optimal long-term storage of excess carbohydrates as fats, and may contribute to the imbalance between nutrient utilization and storage, which is characteristic of obesity. ChREBP represses E-box-dependent transcription forms and forms heterodimers with Mlx to bind the DNA sequence CACGTG. ChREBP is encoded by the WBSCR14 gene, which is located within the Williams-Beuren syndrome (WBS) deletion at chromosome 7q11.23. WBS is a neuro-developmental disorder affecting several systems. Loss of the encoded transcription factor may contribute to the developmental symptoms found in WBS. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of all ChREBP isoforms of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 401-700 mapping within an internal region of ChREBP of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 401-700 mapping within an internal region of ChREBP of mouse origin |
| Entrez Gene | 58805 |
|---|
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.
Application
Western blot analysis of ChREBP expression in HeLa whole cell lysate.| Application Notes | recommended for detection of all ChREBP isoforms of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
