Application
Western blot analysis of Annexin VIII expression in non-transfected: (A) and human Annexin VIII transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Annexin VIII expression in non-transfected: (A) and mouse Annexin VIII transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic localization.Application
Western blot analysis of Annexin VIII expression in JAR whole cell lysate.| Product Name | Annexin VIII Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 121-180 mapping within an internal region of Annexin VIII of human origin |
| Modification Notes | The annexin family is composed of at least ten mammalian genes that encode calcium-binding proteins. The annexin proteins are characterized by a conserved core domain, which binds to phospholipids in a calcium-dependent manner. Annexin family members have been implicated as regulators of such diverse processes as ion flux, endocytosis and exocytosis, and cellular adhesion. Annexin V is ubiquitously expressed at high levels in tissues and cells grown in tissue culture, while Annexin VIII exhibits a more limited distribution. Where coexpressed in the same tissues, Annexin VIII is often expressed at a 100-fold lower level than Annexin V. However, Annexin VIII is preferentially expressed in acute promyelocytic leukemia (APL) cells, which may relate to its role in hematopoietic cell differentiation. At this time it is believed that there are duplicated copies of ANXA8-like genes on human chromosome 10q11 which putatively encode 3 highly similar proteins designated ANXA8L1 and ANXA8L2 (Annexin A8-like 1 and Annexin A8-like 2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Annexin VIII and, to a lesser extent, Annexin V of mouse, rat and human origin by WB, IP, IF and ELISA also recommended for detection of ANXA8L1 and ANXA8L2 of human origin also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 121-180 mapping within an internal region of Annexin VIII of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 121-180 mapping within an internal region of Annexin VIII of human origin |
| Entrez Gene | 244 |
|---|
Application
Western blot analysis of Annexin VIII expression in non-transfected: (A) and human Annexin VIII transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Annexin VIII expression in non-transfected: (A) and mouse Annexin VIII transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic localization.
Application
Western blot analysis of Annexin VIII expression in JAR whole cell lysate.| Application Notes | recommended for detection of Annexin VIII and, to a lesser extent, Annexin V of mouse, rat and human origin by WB, IP, IF and ELISA also recommended for detection of ANXA8L1 and ANXA8L2 of human origin also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
