Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing Golgi localization.Application
Western blot analysis of DNase I expression in Hep G2 whole cell lysate.Application
Western blot analysis of human recombinant DNase I. Kindly provided by Dr. David O' Hagan, Wayne State University School of Medicine and Karmanos Cancer Institute.| Product Name | DNase I Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 106-155 mapping within an internal region of Deoxyribonuclease I of human origin |
| Modification Notes | Internucleosomal DNA fragmentation following the activation of endonucleases is the common end point of apoptosis. DNase I, a Ca2+/Mg2+-dependent endonuclease ubiquitously expressed in mammalian tissues, has been implicated to mediate internucleosomal DNA degradation in human cells undergoing apoptosis (1). DNase I is highly polymorphic, and at least six alleles of DNase I are known (2). DNase II, the ubiquitously expressed acidic deoxyribonuclease, acts downstream of caspase activation and may also induce DNA digestion during apoptosis (3). DNase I cleaves DNA to 5'-phosphodinucleotide and 5'-phospho- oligonucleotide end-products, whereas DNase II cleaves DNA to 3'-phosphomononucleotide and 3'-phosphooligonucleotide end-products. The mechanism by which DNase II cuts DNA is similar to DNase I, which produces nicks rather than double-strand cuts (4,5). DNase II is usually present in cytoplasm of epithelial cells, but it appears concentrated in the nuclei of lens fibers. In contrast, DNase I is always concentrated in nuclei of epithelial and fiber cells (6). The gene encoding DNase II maps to human chromosome 19 (5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Deoxyribonuclease I of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 106-155 mapping within an internal region of Deoxyribonuclease I of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 106-155 mapping within an internal region of Deoxyribonuclease I of human origin |
| Entrez Gene | 1773 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing Golgi localization.
Application
Western blot analysis of DNase I expression in Hep G2 whole cell lysate.
Application
Western blot analysis of human recombinant DNase I. Kindly provided by Dr. David O' Hagan, Wayne State University School of Medicine and Karmanos Cancer Institute.| Application Notes | recommended for detection of Deoxyribonuclease I of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
