Application
Western blot analysis of eIF2Bδ expression in non-transfected: (A) and mouse eIF2Bδ transfected: (B) 293T whole cell lysates.Application
Western blot analysis of eIF2Bδ expression in non-transfected 293T: (A), human eIF2Bδ transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.| Product Name | eIF2Bδ Antibody (H-280) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 224-503 mapping near the C-terminus of eIF2Bδ of human origin |
| Modification Notes | The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF2B exists as a five subunit complex composed of eIF2Bå, eIF2Bß, eIF2B©, eIF2B∂, and eIF2Bé. The eIF2B complex catalyzes the exchange of GDP for GTP on the eIF2 complex, following the interaction of eIF2/GTP with the 40S ribosomal subunit. Guanine nucleotide exchange factor (GEF) activity is exhibited by the eIF2Bé subunit alone, but is greater in the presence of all five eIF2B subunits. Phosphorylation of eIF2 inhibits GEF activity of eIF2B, an inhibition that requires the eIF2Bå subunit. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of eIF2Bδ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 224-503 mapping near the C-terminus of eIF2B&delta of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 224-503 mapping near the C-terminus of eIF2Bδ of human origin |
| Entrez Gene | 8890 |
|---|
Application
Western blot analysis of eIF2Bδ expression in non-transfected: (A) and mouse eIF2Bδ transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of eIF2Bδ expression in non-transfected 293T: (A), human eIF2Bδ transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.| Application Notes | recommended for detection of eIF2Bδ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
