Application
Western blot analysis of HP1 expression in Jurkat nuclear extract.Application
Western blot analysis of HP1α expression in non-transfected 293: (A), human HP1α transfected 293: (B) and K-562 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast cancer tissue showing nuclear staining of tumor cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Product Name | HP1 Antibody (FL-191) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-191 representing full length HP1α of human origin |
| Modification Notes | Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60 and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated Histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1), HP1?, HP1? and HP1?. As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of Histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as Histone H3 is dephosphorylated. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of HP1α, HP1β and HP1γ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-191 representing full length HP1&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-191 representing full length HP1α of human origin |
| Entrez Gene | 23468 |
|---|
Application
Western blot analysis of HP1 expression in Jurkat nuclear extract.
Application
Western blot analysis of HP1α expression in non-transfected 293: (A), human HP1α transfected 293: (B) and K-562 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast cancer tissue showing nuclear staining of tumor cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Application Notes | recommended for detection of HP1α, HP1β and HP1γ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
