Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of TIRAP expression in non-transfected: (A) and human TIRAP transfected: (B) 293T whole cell lysates.Application
Western blot analysis of TIRAP expression in non-transfected: (A) and human TIRAP transfected: (B) 293T whole cell lysates.| Product Name | TIRAP Antibody (FL-235) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-235 representing full length TIRAP of human origin |
| Modification Notes | Mammalian Toll-like receptors (TLRs) recognize conserved products of microbial metabolism and activate NF˚B and other signaling pathways through the adapter protein MyD88. MyD88 functions as an adapter protein in the association of IL-1 receptor associated kinase (IRAK) with the IL-1 receptor. MyD88 contains a characteristic N-terminal death domain, which is essential for NF˚B activation, and an adjacent Toll/Il-1R homology domain (TIR domain), which is responsible for signal transduction. TIR domain-containing adapter protein (TIRAP), also designated MAL (MyD88 adapter-like), wyatt or TLR-4 adaptor protein, is a cytoplasmic TIR-domain-containing protein that activates NF˚B, Jun N-terminal kinase and extracellular signal-regulated kinase-1 and -2. TIRAP forms homodimers and heterodimers with MyD88. IRAK-2, but not IRAK, is required for the activation of NF˚B by TIRAP which associates with IRAK-2 through the TIR domain. In addition, TIRAP associates with TLR-4, suggesting that it plays a role in TLR-4 signal transduction. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of TIRAP of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-235 representing full length TIRAP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-235 representing full length TIRAP of human origin |
| Entrez Gene | 114609 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of TIRAP expression in non-transfected: (A) and human TIRAP transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of TIRAP expression in non-transfected: (A) and human TIRAP transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of TIRAP of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
