Application
Western blot analysis of human recombinant AQP2 fusion protein.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human renal cancer tissue showing perinuclear and cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of AQP2 expression in Caki-1 (A) and COLO 320DM (B) whole cell lysates.| Product Name | AQP2 Antibody (H-40) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 232-271 mapping within a C-terminal cytoplasmic domain of AQP2 of human origin |
| Modification Notes | Aquaporins (AQPs) are a large family of integral membrane water transport channel proteins that facilitate the transport of water through the cell membrane. This function is conserved in animals, plants and bacteria. Many isoforms of aquaporin have been identified in mammals, designated AQP0 through AQP10. Aquaporins are widely distributed and it is not uncommon for more than one type of AQP to be present in the same cell. Although most aquaporins are only permeable to water, AQP3, AQP7, AQP9 and one of the two AQP10 transcripts are also permeable to urea and glycerol. AQP2 is the only water channel that is activated by vasopressin to enhance water reabsorption in the kidney collecting duct. Aquaporins are involved in renal water absorption, generation of pulmonary secretions, lacrimation, and the secretion and reabsorption of cerebrospinal fluid and aqueous humor. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of AQP2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 232-271 mapping within a C-terminal cytoplasmic domain of AQP2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 232-271 mapping within a C-terminal cytoplasmic domain of AQP2 of human origin |
| Entrez Gene | 359 |
|---|
Application
Western blot analysis of human recombinant AQP2 fusion protein.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human renal cancer tissue showing perinuclear and cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of AQP2 expression in Caki-1 (A) and COLO 320DM (B) whole cell lysates.| Application Notes | recommended for detection of AQP2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
