Application
Western blot analysis of HoxA1 expression in non-transfected: (A) and human HoxA1 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Product Name | HoxA1 Antibody (H-130) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 76-205 mapping within an internal region of HoxA1 of human origin |
| Modification Notes | Hox genes play a fundamental role in the development of the vertebrate central nervous system, heart, axial skeleton, limbs, gut, urogenital tract and external genitalia. The homeobox gene HoxA1 is transcriptionally regulated by retinoic acid (RA) and encodes a transcription factor which has been shown to play important roles in cell differentiation and embryogenesis. HoxA1 is also expressed in cancers, such as mammary tumors, though it is not expres-sed in normal gland or in precancerous mammary tissues. At embryonic stages, HoxA2 is expressed in the mesenchyme and epithelial cells of the palate, however its expression is restricted to the tips of the growing palatal shelves. HoxA2 protein is predominantly expressed in the nuclei of cells in the ventral mantle region of the developing embryo. In the developing and adult mouse spinal cord, HoxA2 protein may contribute to dorsal-ventral patterning and/or to the specification of neuronal phenotype. HoxA7 functions as a potent transcriptional repressor and its action as such requires several domains, including both activator and repressor regions. HoxA7 is expressed in the fetal liver, lung, skeletal muscle, kidney, pancreas and placenta. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of HoxA1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 76-205 mapping within an internal region of HoxA1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 76-205 mapping within an internal region of HoxA1 of human origin |
| Entrez Gene | 3198 |
|---|
Application
Western blot analysis of HoxA1 expression in non-transfected: (A) and human HoxA1 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Application Notes | recommended for detection of HoxA1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
