Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomeruli and tubules.Application
Western blot analysis of MRCL3/MRLC2/MYL9 expression in BC3H1 (A) and A-10 (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed A-10 cells showing cytoplasmic localization.Application
Western blot analysis of MRCL3/MRLC2/MYL9 expression in A-10 whole cell lysate.| Product Name | MRCL3/MRLC2/MYL9 Antibody (FL-172) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-172 representing the full length myosin regulatory light chain encoded by MRCL3 of human origin |
| Modification Notes | Myosin is a highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements. Conventional myosins are hexameric proteins consisting of two heavy chain subunits, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits. Three general classes of myosin have been cloned: smooth muscle myosins, striated muscle myosins and non-muscle myosins. Myosin regulatory light chains, including MRCL3 (also known as MRLC3 or MLCB), MRLC2 (also known as MLC-B) and MYL9 (also known as LC20, MLC2, MRLC1 or MYRL2), regulate contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK). Phosphorylation of myosin regulatory light chains, catalyzed by MLCK in the presence of calcium and calmodulin, increases the Actin-activated myosin ATPase activity, thereby regulating the contractile activity. Myosin light chain is also located in striated skeletal muscle, where its function remains undefined. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of myosin regulatory light chains encoded by MRCL3, MRLC2 and MYL9, and LOC391722 protein of human origin Mylc2b, Myl9 and Myl12a of mouse origin and Mrlcb and Myl9 of rat origin WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-172 representing the full length myosin regulatory light chain encoded by MRCL3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-172 representing the full length myosin regulatory light chain encoded by MRCL3 of human origin |
| Entrez Gene | 103910 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomeruli and tubules.
Application
Western blot analysis of MRCL3/MRLC2/MYL9 expression in BC3H1 (A) and A-10 (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed A-10 cells showing cytoplasmic localization.
Application
Western blot analysis of MRCL3/MRLC2/MYL9 expression in A-10 whole cell lysate.| Application Notes | recommended for detection of myosin regulatory light chains encoded by MRCL3, MRLC2 and MYL9, and LOC391722 protein of human origin Mylc2b, Myl9 and Myl12a of mouse origin and Mrlcb and Myl9 of rat origin WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
