Application
Western blot analysis of ADAM9 expression in WEHI-3 whole cell lysate.Application
Western blot analysis of ADAM9 expression in Caki-1 whole cell lysate.| Product Name | ADAM9 Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 35-94 mapping within an N-terminal extracellular domain of ADAM9 of human origin |
| Modification Notes | The human ADAM9 gene maps to chromosome 8p11 and encodes an 819 amino acid glycoprotein that is present in brain, liver, heart, kidney, lung, and trachea (1–3). ADAM (a disintegrin and metalloprotease) glycoproteins are a family of over 30 membrane-anchored, Zn2+-dependent proteases that influence fertilization, muscle fusion, cytokine secretion, modulation of Notch-related neurogenic pathways, monocyte fusion, and many other cell adhesion-dependent events (4–6). ADAM proteins contain a signal domain, a pro domain, a metalloprotease domain, a disintegrin domain (Integrin ligand), a cysteine-rich region, an epidermal growth factor-like domain, a transmembrane (TM) domain (alternative splicing before the TM domain in ADAM11, 12, 17, and 28 can yield soluble forms), and a cytoplasmic tail (4–8). Removal of the amino-terminal signal peptide initiates secretion from the cell, or anchoring on the cell surface (3,4–6). Furin or furin-like proprotein convertase-dependent cleavage of the pro domain initiates catalytic activity of the metalloprotease (3,4–6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of precursor and mature ADAM9 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 35-94 mapping within an N-terminal extracellular domain of ADAM9 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 35-94 mapping within an N-terminal extracellular domain of ADAM9 of human origin |
| Entrez Gene | 8754 |
|---|
Application
Western blot analysis of ADAM9 expression in WEHI-3 whole cell lysate.
Application
Western blot analysis of ADAM9 expression in Caki-1 whole cell lysate.| Application Notes | recommended for detection of precursor and mature ADAM9 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
