Application
Western blot analysis of Cytokeratin 18 expression in HeLa whole cell lysate.Application
Western blot analysis of Cytokeratin 18 expression in HeLa (A), Hep G2 (B), MCF7 (C), SKBR-3 (D) and T24 (E) whole cell lysates.Application
Western blot analysis of Cytokeratin 18 expression in SK-BR-3 whole cell lysate.Application
Western blot analysis of Cytokeratin 18 expression in Hep G2 whole cell lysate.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.Application
Western blot analysis of Cytokeratin 18 expression in T24 (A) and MCF7 (B) whole cell lysates.| Product Name | Cytokeratin 18 Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-80 mapping at the N-terminus of Cytokeratin 18 of human origin |
| Modification Notes | Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. For example, Cytokeratins 10 and 13 are expressed highly in a subset of squamous cell carcinomas while Cytokeratin 18 is expressed in a majority of adenocarcinomas and basal cell carcinomas. Cytokeratin 18 contains two major phosphorylation sites on Ser 33 and Ser 52. Phosphorylation of Ser 18 is essential for the association of Cytokeratin 18 with 14-3-3 proteins and is involved in keratin organization and distribution. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Cytokeratin 18 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-80 mapping at the N-terminus of Cytokeratin 18 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-80 mapping at the N-terminus of Cytokeratin 18 of human origin |
| Entrez Gene | 3875 |
|---|
Application
Western blot analysis of Cytokeratin 18 expression in HeLa whole cell lysate.
Application
Western blot analysis of Cytokeratin 18 expression in HeLa (A), Hep G2 (B), MCF7 (C), SKBR-3 (D) and T24 (E) whole cell lysates.
Application
Western blot analysis of Cytokeratin 18 expression in SK-BR-3 whole cell lysate.
Application
Western blot analysis of Cytokeratin 18 expression in Hep G2 whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.
Application
Western blot analysis of Cytokeratin 18 expression in T24 (A) and MCF7 (B) whole cell lysates.| Application Notes | recommended for detection of Cytokeratin 18 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
