Application
Western blot analysis of α Tubulin expression in non-transfected: (A) and mouse α Tubulin transfected: (B) 293T whole cell lysates.Application
Western blot analysis of α1a Tubulin expression in non-transfected 293T: (A), human α1a Tubulin transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of α3C Tubulin expression in non-transfected 293T: (A), human α3C Tubulin transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of α Tubulin expression in non-transfected 293: (A), human α Tubulin transfected 293: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of α Tubulin expression in non-transfected: (A) and mouse α Tubulin transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor (A) and immunofluorescence staining of methanol-fixed HeLa cells (B) showing cytoplasmic localization.| Product Name | α Tubulin Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 149-448 of α Tubulin of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | Tubulin is a major cytoskeleton component that has five distinct forms, designated a, b, g, d and e tubulin. a and b tubulins form heterodimers which multimerize to form a microtubule filament. Multiple b Tubulin isoforms (b1, b2, b3, b4, b5, b6 and b8) have been characterized and are expressed in mammalian tissues. b1 and b4 are present throughout the cytosol, b2 is present in the nuclei and nucleoplasm, and b3 is a neuron-specific cytoskeletal protein. g Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both d Tubulin and e Tubulin are associated with the centrosome. d Tubulin is a homolog of the Chlamydomonas d Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell cycle-specific pattern of localization; first associating with only the older of the centrosomes in a newly duplicated pair, and later associating with both centrosomes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| WB:1:100~1:400 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | zebrafish, Drosophila ,Xenopus,equine, canine, bovine and porcine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 149-448 of α Tubulin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 149-448 of α Tubulin of human origin |
Application
Western blot analysis of α Tubulin expression in non-transfected: (A) and mouse α Tubulin transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of α1a Tubulin expression in non-transfected 293T: (A), human α1a Tubulin transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of α3C Tubulin expression in non-transfected 293T: (A), human α3C Tubulin transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of α Tubulin expression in non-transfected 293: (A), human α Tubulin transfected 293: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of α Tubulin expression in non-transfected: (A) and mouse α Tubulin transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor (A) and immunofluorescence staining of methanol-fixed HeLa cells (B) showing cytoplasmic localization.| Application Notes | WB:1:100~1:400 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
