Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bone marrow tissue showing cytoplasmic staining of megakaryocytes and other hematopoietic cells.| Product Name | PF-4 Antibody (H-40) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 27-66 mapping at the N-terminus of PF-4 of human origin |
| Modification Notes | Platelet factor-4 (PF-4 or PF4) is a 70 amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Platelets secrete low-molecular-weight PF-4, which binds to and neutralizes heparin and related sulfated glycosaminoglycans (GAGs). Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF-4 probably has a role in inflammation and wound repair. Both PF4 and eotaxin, a specific chemoattractant for eosinophils, have been shown to exhibit stronger expression in spleens of adult NOA mice (an animal model of allergic or atopic dermatitis) than in younger mice, parallel to the increase in ulcerative skin lesions in older mice. This suggests that PF4 and eotaxin may play important roles in the etiology of atopic dermatitis. PF-4 is encoded by a small inducible gene (SIG), so called because of its small size and its stimulation with platelet activation. The gene which encodes PF-4 maps to human chromosome 4q13.3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of precursor and mature chain of PF-4 of human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 27-66 mapping at the N-terminus of PF-4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 27-66 mapping at the N-terminus of PF-4 of human origin |
| Entrez Gene | 5196 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bone marrow tissue showing cytoplasmic staining of megakaryocytes and other hematopoietic cells.| Application Notes | recommended for detection of precursor and mature chain of PF-4 of human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
