Application
Western blot analysis of phosphorylated JNK expression in untreated (A) and UV-treated (B) 293 cell extracts.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear and cytoplasmic localization.| Product Name | p-JNK Antibody (Thr 183) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against a short amino acid sequence containing phosphorylated Thr 183 of JNK of human origin |
| Conjugation | Agarose |
| Modification | Phosphoralated |
| Modification Notes | JNKs (c-Jun N-terminal kinases) belong to a family of MAP kinases that are involved in a variety of cellular processes, including transcriptional regulation and cellular proliferation, differentiation and development. JNK2 (c-Jun N-terminal kinase 2) and JNK3 (c-Jun N-terminal kinase 3) are 424 and 464 amino acid proteins, respectively, that each contain one protein kinase domain and use magnesium as a cofactor to catalyze the phosphorylation of target proteins, thereby playing a role in a variety of events throughout the cell. Both JNK2 and JNK3 exist as multiple alternatively spliced isoforms and are subject to post-translational phosphorylation on Thr 183 and Thr 221, respectively, an event which activates JNK2/JNK3 enzymatic activity. Defects in the gene encoding JNK3 are a cause of epileptic encephalopathy of the Lennox-Gastaut type, a group of epileptic disorders characterized by severe psychomotor delay and seizures. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHCIPWB |
| WB:1:100~1:500 IP:1:20~1:100 IF:1:5~1:20 IHC:1:10~1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against a short amino acid sequence containing phosphorylated Thr 183 of JNK of human origin |
|---|---|
| Tissue Specificity | raised against a short amino acid sequence containing phosphorylated Thr 183 of JNK of human origin |
| Entrez Gene | 5601 |
|---|
Application
Western blot analysis of phosphorylated JNK expression in untreated (A) and UV-treated (B) 293 cell extracts.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear and cytoplasmic localization.| Application Notes | WB:1:100~1:500 IP:1:20~1:100 IF:1:5~1:20 IHC:1:10~1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
