Application
Western blot analysis of Rad1 expression in non-transfected: (A) and human Rad1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Rad1 expression in HeLa (A), K-562 (B) and Jurkat (C) whole cell lysates.| Product Name | Rad1 Antibody (FL-282) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-282 representing full length Rad1 of human origin |
| Modification Notes | DNA damage or incomplete replication of DNA results in inhibition of cell cycle progression at the G1-S or G2-M checkpoints by conserved regulatory mechanisms. Rad17 is involved in regulation of cell cycle arrest at the G1 checkpoint, whereas Chk1, Rad1, Rad9 and Hus1 are involved in regulation of cell cycle arrest at the G2 checkpoint. Over- expression of Rad17 results in p53 activation and an accumulation of cells in G1 phase. Chk1 functions as an essential component in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage, thus inhibiting mitosis. Hus1 and Rad9 exhibit conserved function in fission yeast and higher eukaryotes. Hus1 has been shown to be phosphorylated in response to DNA damage, a process which requires rad checkpoint genes. Rad9 is thought to be a candidate tumor sup-pressor gene because it is localized to a region of human chromosome 11 containing a number of tumor suppressor loci. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Rad1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-282 representing full length Rad1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-282 representing full length Rad1 of human origin |
| Entrez Gene | 5810 |
|---|
Application
Western blot analysis of Rad1 expression in non-transfected: (A) and human Rad1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Rad1 expression in HeLa (A), K-562 (B) and Jurkat (C) whole cell lysates.| Application Notes | recommended for detection of Rad1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
