Application
Western blot analysis of NF-1 expression in HeLa nuclear extract.Application
Western blot analysis of NF-1 expression in non-transfected: (A) and mouse NF-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of NF-1 expression in nuclear extracts prepared from PMA-treated HeLa (A), untreated Jurkat (B) and PMA-treated NIH/3T3 (C) cells.| Product Name | NF-1 Antibody (N-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of NF-1 of human origin |
| Modification Notes | NF-1, also designated CTF, consists of a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain, and activates transcription through a putatively novel, proline-rich, carboxy terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, å-globin, HSP 70, GRP 78, Histone H1, myelin basic protein and in the Xenopus laevis vitellogenin gene promoter. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of all NF-1 isoforms of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of NF-1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of NF-1 of human origin |
| Entrez Gene | 4774 |
|---|
Application
Western blot analysis of NF-1 expression in HeLa nuclear extract.
Application
Western blot analysis of NF-1 expression in non-transfected: (A) and mouse NF-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of NF-1 expression in nuclear extracts prepared from PMA-treated HeLa (A), untreated Jurkat (B) and PMA-treated NIH/3T3 (C) cells.| Application Notes | recommended for detection of all NF-1 isoforms of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
