Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of Leydig cells and cells in seminiferus ducts (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immuno?fluorescence staining of methanol-fixed HeLa cells showing cytoplasmic local?ization.WB
(A) SIRT5 protein expression in ENKTL and NNM tissues determined by western blotting.IHC
(B) SIRT5 and GPI protein expression in ENKTL and NNM tissues measured by IHC.WB
(H) SIRT5 and GPI protein levels were detected by western blotting.WB
(B) SIRT5 and GPI protein levels in the tumor tissues were analyzed by western blotting.| Product Name | GPI Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Glucose-6-phosphate isomerase (GPI) has many other names, including Phosphohexose isomerase (PHI), Neuroleukin (NLK) and Spermantigen-36 (SA-36). GPI is a cytoplasmic homodimer belonging to the GPI family. It is a neurotrophic factor for spinal and sensory neurons and is involved in glycolysis and gluconeogenesis. Defects or mutations in GPI can cause hereditary nonspherocytic hemolytic anemia (HA), hydrops fetalis, immediate neonatal death and neurological impairment. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| recommended for detection of GPI of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of GPI of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of GPI of human origin |
| Entrez Gene | 2821 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of Leydig cells and cells in seminiferus ducts (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immuno?fluorescence staining of methanol-fixed HeLa cells showing cytoplasmic local?ization.
WB
(A) SIRT5 protein expression in ENKTL and NNM tissues determined by western blotting.
IHC
(B) SIRT5 and GPI protein expression in ENKTL and NNM tissues measured by IHC.
WB
(H) SIRT5 and GPI protein levels were detected by western blotting.
WB
(B) SIRT5 and GPI protein levels in the tumor tissues were analyzed by western blotting.| Application Notes | recommended for detection of GPI of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
