Application
Western blot analysis of TTC25 expression in non-transfected: (A) and human TTC25 transfected: (B) 293T whole cell lysates.| Product Name | TTC25 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 181-480 mapping within an internal region of TTC25 of human origin |
| Modification Notes | TTC25 (tetratricopeptide repeat protein 25) is a 672 amino acid protein that contains eight TPR repeats and exists as two alternatively spliced isoforms. The gene that encodes TTC25 consists of approximately 30,782 bases and maps to human chromosome 17q21.2. Encoding more than 1,200 genes, chromosome 17 comprises over 2.5% of the human genome. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and Li-Fraumeni syndrome. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of ovary, colon, prostate gland and fallopian tubes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of TTC25 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 181-480 mapping within an internal region of TTC25 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 181-480 mapping within an internal region of TTC25 of human origin |
| Entrez Gene | 83538 |
|---|
Application
Western blot analysis of TTC25 expression in non-transfected: (A) and human TTC25 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of TTC25 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
