Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.Application
Western blot analysis of GILZ expression in non-transfected: (A) and human GILZ transfected: (B) 293T whole cell lysates.Application
Western blot analysis of GILZ expression in non-transfected: (A) and mouse GILZ transfected: (B) 293T whole cell lysates.Application
Western blot analysis of GILZ expression in non-transfected: (A) and human GILZ transfected: (B) 293T whole cell lysates.Application
Western blot analysis of GILZ expression in non-transfected: (A) and human GILZ transfected: (B) 293 whole cell lysates.| Product Name | GILZ Antibody (FL-134) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-134 representing full length GILZ of human origin |
| Modification Notes | Glucocorticoid-induced leucine zipper (GILZ) is a leucine zipper protein ex-pressed in normal lymphocytes from thymus, spleen and lymph nodes. It is absent in nonlymphoid tissues including brain, liver and kidney. GILZ mediates the immunosuppressive effects of glucocorticoid hormones; its expression is induced in T cells by dexamethasone. GILZ protects T cells from an anti-CD3 antibody-induced apoptosis by inhibiting Fas and Fas ligand expression. It interferes with Egr-2, Egr-3, NFAT/AP-1-inducible transcription factors and AP-1. The interaction of GILZ with c-Fos and c-Jun inhibits the binding of active AP-1 to its DNA consensus site in vitro. GILZ also binds NFkB subunits and inhibits the NFkB nuclear translocation. It inhibits T cell receptor-induced interleukin-2/interleukin-2 receptor expression. The binding of GILZ to Raf-1 prevents Raf-MEK-ERK activation in the MAPK pathway. GILZ is expressed by normal macrophages in nonlymphoid tissues and by tumor-infiltrating macrophages in Burkitt lymphomas. The gene encoding human GILZ maps to chromosome Xq22.3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GILZ and all other TSC22 family members of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-134 representing full length GILZ of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-134 representing full length GILZ of human origin |
| Entrez Gene | 1831 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.
Application
Western blot analysis of GILZ expression in non-transfected: (A) and human GILZ transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of GILZ expression in non-transfected: (A) and mouse GILZ transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of GILZ expression in non-transfected: (A) and human GILZ transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of GILZ expression in non-transfected: (A) and human GILZ transfected: (B) 293 whole cell lysates.| Application Notes | recommended for detection of GILZ and all other TSC22 family members of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
