Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.| Product Name | NNT-1/BSF-3 Antibody (FL-225) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-225 representing full length NNT-1/BSF-3 of human origin |
| Modification Notes | Neurotrophin-1/B cell-stimulating factor-3 (NNT-1/BSF-3, also known as cardiotrophin-like cytokine) is found mainly in lymph nodes and spleen (1). NNT-1/BSF-3 induces tyrosine phosphorylation of the signal transducing receptor molecule glycoprotein 130 (gp130), leukemia inhibitory factor receptor beta, and signal transducer and activator of transcription 3 in the SK-N-MC human neuroblastoma cells (1). The activation of gp130 distinguishes a group of cytokines referred to as the IL-6 family (2-4). They all show the conserved location of one intron in their gene structure and, in common with cytokines of the hematopoietin superfamily, the presence of a four-helix bundle in their protein structure (5,6). In addition to features typical of IL-6 family cytokines, including neurotropic effects, NNT-1/BSF-3 shows B cell-stimulating capability (1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NNT-1/BSF-3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-225 representing full length NNT-1/BSF-3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-225 representing full length NNT-1/BSF-3 of human origin |
| Entrez Gene | 23529 |
|---|
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.| Application Notes | recommended for detection of NNT-1/BSF-3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
