Application
Western blot analysis of PSGL-1 expression in non-transfected: (A) and human PSGL-1 transfected: (B) 293 whole cell lysates.| Product Name | PSGL-1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 113-412 mapping at the C-terminus of PSGL-1 of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | PSGL-1 (P-Selectin glycoprotein ligand, also designated CD162), exists as a disulfide-linked homodimer. PSGL-1 is a type 1 membrane protein that localizes on the tips of microvilli of leukocytes. Its extracellular domain is rich in serines, threonines and prolines, and includes a series of 15 and 16 decameric repeats in HL-60 and U-937 cells, and human leukocytes, respectively. Although PSGL-1 appears to be the sole receptor for P-Selectin on human hematopoietic cells, it also interacts with E-Selectin through a unique binding site. In order to bind PSGL-1 to either E-Selectin or P-Selectin, PSGL-1 must be sialylated and fucosylated. PSLG-1 is a mucin-like molecule, much like leukosialin (CD43), CD164 and CD34. These proteins belong to an emerging family of cell adhesion receptors called sialomucins, which transduce negative signals in hematopoietic cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| recommended for detection of PSGL-1 of human origin by WB, IP,IHC, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 113-412 mapping at the C-terminus of PSGL-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 113-412 mapping at the C-terminus of PSGL-1 of human origin |
| Entrez Gene | 6404 |
|---|
Application
Western blot analysis of PSGL-1 expression in non-transfected: (A) and human PSGL-1 transfected: (B) 293 whole cell lysates.| Application Notes | recommended for detection of PSGL-1 of human origin by WB, IP,IHC, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
