Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of Islets of Langerhans.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear, cytoplasmic and membrane localization.Application
Western blot analysis of V-ATPase A1 expression in SK-N-MC whole cell lysate.Application
Western blot analysis of V-ATPase A1 expression in non-transfected: (A) and human V-ATPase A1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of V-ATPase A1 expression in non-transfected: (A) and mouse V-ATPase A1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of V-ATPase A1 expression in non-transfected: (A) and human V-ATPase A1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of V-ATPase A1 expression in 293T whole cell lysate.| Product Name | V-ATPase A1 Antibody (H-140) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 71-210 mapping within an extracellular domain of V-ATPase A1 of human origin |
| Modification Notes | The subunit of the vacuolar proton pump is a V-ATPase that has two different isoforms. The type I isoform contains an 18-base pair insert and is expressed in brain, whereas the truncated type II isoform is more widely expressed, including lung, kidney and spleen. The subunit of the vacuolar proton pump is located in clathrin-coated vesicles and is also found in osteoclasts. It consists of two fundamental domains, a hydrophilic amino-terminus, which has greater than 30% charged residues, and a hydrophobic carboxy terminus, which contains at least six transmembrane regions. The proton pump functions in coupling ATP hydrolysis by the cytoplasmic subunits to proton translocation by the intramembranous components of the pump. The inactivation of the osteoclast-specific vacuolar proton ATPase subunit is responsible for the lack of the enzyme in the apical membranes of osteoclast cells in osteosclerotic mutant mice, thus preventing the resorption function of these cells and leading to the osteopetrotic phenotype. The subunit, which co-localizes with the late endosomal marker Rab7 on vacuolar membranes, is essential for vacuole formation by selective swelling of late endosomes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of V-ATPase A1 and, to a lesser extent, V-ATPase A4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 71-210 mapping within an extracellular domain of V-ATPase A1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 71-210 mapping within an extracellular domain of V-ATPase A1 of human origin |
| Entrez Gene | 535 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of Islets of Langerhans.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear, cytoplasmic and membrane localization.
Application
Western blot analysis of V-ATPase A1 expression in SK-N-MC whole cell lysate.
Application
Western blot analysis of V-ATPase A1 expression in non-transfected: (A) and human V-ATPase A1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of V-ATPase A1 expression in non-transfected: (A) and mouse V-ATPase A1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of V-ATPase A1 expression in non-transfected: (A) and human V-ATPase A1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of V-ATPase A1 expression in 293T whole cell lysate.| Application Notes | recommended for detection of V-ATPase A1 and, to a lesser extent, V-ATPase A4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
