| Product Name | FCP1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 662-961 mapping at the C-terminus of FCP1 of human origin |
| Modification Notes | RNA polymerase II (RNAP II) comprises a 12 subunit protein complex that mediates transcription. RNAP II contains a unique carboxy terminal domain (CTD), which consists of 52 repeats of the consensus heptapeptide Tyr-Ser-Pro-Thr-Ser-Pro-Ser. The RNAP II isoform with an unphosphorylated CTD associates with transcription initiation complexes, whereas the isoform with a phosphorylated CTD is involved in transcription elongation. FCP1, also designated TFIIF-associated CTD phosphatase, is a protein phosphatase dedicated to the CTD of RNAP II that mediates the recycling of RNAP II during the transcription cycle. FCP1 specifically targets phosphorylated CTD serine 2 and 5 with similar affinities. Phosphorylation of FCP1 itself is necessary for sufficient activity of the protein and its interaction with TFIIF. Upon activation, the a-helical carboxy terminus of FCP1 binds to RAP74 to form a complex. FCP1 also represses HIV-1 Tat-mediated transactivation, and, therefore, may represent a specific target for modulation of Tat activity in infected cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of FCP1, FCP1a and FCP1b of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 662-961 mapping at the C-terminus of FCP1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 662-961 mapping at the C-terminus of FCP1 of human origin |
| Entrez Gene | 9150 |
|---|
| Application Notes | recommended for detection of FCP1, FCP1a and FCP1b of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
